Toxins

Christian Som 

Christian has provided the following abstract for the technically minded.

Neotropical poison frogs (Dendrobatidae) contain a wide variety of lipophilic alkaloids, apparently accumulated unchanged into skin glands from dietary sources. Panamanian poison frogs, Dendrobates azureus, raised in a large, screened, outdoor cage and provided for six months with leaf litter from the frog’s natural habitat, accumulated a variety of alkaloids into the skin.

These included:

two isomers of the ant pyrrolizidine 251K;

two isomers of the 3,5-disubstituted indolizidine 195B;

an alkaloid known to occur in myrmicine ants; another such indolizidine, 211E;

two pyrrolidines, 197B and 223N, the former known to occur in myrmicine ante;

two tricyclics, 193C and 2191, the former known to occur as precoccinelline in coccinellid beetles; and

three spiropyrrolizidines, 222, 236, and 252A, representatives of an alkaloid class known to occur in millipedes.

The alkaloids 211E, 197B, and 223N appear likely to derive in part from ants that entered the screened cage.

In addition, the frog skin extracts contained trace amounts of four alkaloids, 205D, 207H, 219H, and 231H, of unknown structures and source. Wild caught frogs from the leaf litter site contained nearly 40 alkaloids, including most of the above alkaloids.

Pumiliotoxins and histrionicotoxins were major alkaloids in wildcaught frogs, but were absent in captive-raised frogs. Ants microsympatric with the poison frogs at the leaf-litter site and at an island site nearby in the Bay of Panamá were examined for alkaloids. The decahydroquinoline 195A and two isomers of the pyrrolizidine 251K were found to be shared by microsympatric myrmicine ants and poison frogs.

The proportions of the two isomers of 251K were the same in ant and frog.

Reference:
Arthropod-frog connection: Decahydroquinoline and pyrrolizidine alkaloids common to microsympatric myrmicine ants and dendrobatid frogs. Daly JW, Garraffo HM, Jain P, Spande TF, Snelling RR, Jaramillo C, Rand AS Journal of Chemical Ecology 26: (1) 73-85 Jan 2000