Drosophila hydei
This, the large black species, can be fickle to produce with cultures often failing.  Dr Shin Sugiyama, has suggested that one of the problems seems to be that: male adult hydei take a feast a week after emerging to become sexually mature  This would probably effectively prevent mating between siblings in the wild.  Because the females are quicker the solution appears to be the mixing of old males (+10 days) with new females to get good egg production that can be done by simply mixing old and new flies.  Shin no longer maintains this species but would appreciate the results of any experiments carried out. Shin Sugiyama